Overview

Description

BaseScope™ Reagent Kit v2 - RED Assay is based on ACD"s patented signal amplification and background suppression technology. This high sensitive RNA ISH technique and can be used for short targets and splice variants. The BaseScope™ Reagent Kit v2- RED assay includes the Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is expected ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield. Microscope .Each kit contains the BaseScope Detection Kit v2 (Cat# 323910), RNAscopeﾮ Pretreat Reagents-H202 and Protease Reagents (Cat#322381), RNAscope Target Retrieval (Cat#322000), RNAscope Wash Buffer (Cat#310091).

RNAScope Workflow

• Permeabilize
• Hybridize
• Amplify
• Visualize
• 

Specifications

Shipping temperature(°C):	4 ﾰC
Storage temperature(°C):	4 ﾰC
Shelf life:	9 months from the date of manufacturing.

• * Please check expiration dates on the reagent package

Product Documents

Product Documents

acdbio的ACD组织库

ACD精选了数十种预先来源的经过验证的和未经验证的组织块，供我们的PAS客户使用，以支持ISH探针和测定的开发。组织库包含：

多种正常和癌变的人体组织

一套完整的食蟹猴组织，有雄性和雌性的复制品

小鼠和大鼠的多器官组织阵列

HPV控制细胞沉淀阵列

控制HeLa细胞沉淀

我们的组织库使这套高质量的样本易于获取，并且种类丰富，可确保立即提供用于验证和优化ISH探针和测定的合适组织。

节省时间和成本

从ACD组织库组织样本可以直接在PAS研究使用，无需验证，并提供自己的组织样本，或从3源和购买样品RD第三方供应商，否则可能需要2-3周。

无需对未经测试的组织进行QC检测，我们经过验证的组织样品的合格率为100％，并被证明具有良好的RNA质量。我们的PAS科学家还优化了这些样品的预处理条件，以确保成功使用RNAscope，从而简化了探针和测定开发流程。